Coding
Part:BBa_K4606004:Experience
Designed by: Baochao Li Group: iGEM23_Nanjing-NFLS (2023-09-14)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
Applications of BBa_K4606004
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
User Reviews
UNIQ905c95d9aea604f8-partinfo-00000000-QINU UNIQ905c95d9aea604f8-partinfo-00000001-QINU