Coding
Part:BBa_K4606004:Experience
Designed by: Baochao Li Group: iGEM23_Nanjing-NFLS (2023-09-14)
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
For detailed design information, you can look up the registry of (BBa_K 4606003), the device for gene segment of SMAD4.
Applications of BBa_K4606004
According to the base sequence information of SMAD4 in the database, we mutated the SMAD4 gene in a region that complementary binds to miR-3074-5p. By inserting its 3’UTR region (414bp) behind the luciferase gene in the vector, we could determine the expression level of this gene segment.
User Reviews
UNIQ8127ff5799fe908a-partinfo-00000000-QINU UNIQ8127ff5799fe908a-partinfo-00000001-QINU